WebOct 30, 2015 · Chloroplast gene expression is shown as the value of log2 (FPKM). Gene expression and RNA editing sites were matched to the genomic position, whereas the bars for editing efficiency (e.g ndhD) were shifted due to multiple editing sites within very narrow windows. The ndhB gene (15 sites) was thus drawn in a separate window. Total DNAs or RNAs were extracted from cultured cells in liquid medium and transgenic calli using a DNeasy Plant Mini Kit or RNeasy Plant Mini Kit (Qiagen). Cultured cells and calli were harvested by centrifugation at 10,000 r.p.m. for 1 min. The cDNA from total RNA was reverse-transcribed using RNA to … See more DdCBE Golden Gate destination vectors were constructed using Gibson assembly (New England Biolabs). Sequences encoding the TAL N-terminal domain, HA tag, FLAG tag, TAL … See more DdCBE DNA templates were prepared by PCR using Phusion High-Fidelity DNA Polymerase (Thermo Scientific). DdCBE mRNAs were synthesized and purified using an in vitro mRNA synthesis kit (Enzynomics). The … See more Lettuce protoplasts transfected with DdCBE-encoding plasmids were resuspended in lettuce protoplast culture medium19 (LPCM) … See more Lettuce (Lactuca sativa cv. Cheongchima) seeds were surface sterilized in 70% ethanol for 30 s and in a 0.4% hypochlorite solution for 15 min and were washed three times in distilled water. The lettuce seeds were germinated on … See more
Genome-wide identification of RNA editing sites in chloroplast ...
WebJan 12, 2024 · Our DdCBEs induced base editing in lettuce or rapeseed calli at frequencies of up to 25% (mitochondria) and 38% (chloroplasts). We also showed DNA-free base … WebFeb 9, 2024 · Generally, there is mounting evidence that PPR proteins are involved in the post-transcriptional regulation of chloroplast and/or mitochondrial genes, including RNA maturation, editing, intron splicing, transcripts’ stabilization, and translation initiation. roll with the flava
Plants Free Full-Text Transcriptomics and Metabolomics Analysis ...
WebApr 2, 2024 · It is generally accepted that chloroplast gene expression is largely controlled and regulated at the posttranscriptional and, especially, the translational levels, contrasting gene expression in cyanobacteria, which is mainly transcriptionally regulated. ... Editing of a chloroplast mRNA by creation of an initiation codon. Nature 353: 178–180 ... WebAug 24, 2024 · During evolution, chloroplasts have retained core components of the gene-expression apparatus from their prokaryotic progenitors. In addition, they obtained many … WebIn the future, newly modified CRISPR/Cas9-based approaches may be useful for chloroplast gene editing. PPR proteins with specific sequences can be artificially … roll with stub roll