2024 Laht150

Laht150

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August undefined, 2024

Tīmeklis2024. gada 22. jūn. · LahT150 cleavage was performed by adding LahT150 at a 1:10 ratio to EmbA, and incubating at room temperature overnight for small-scale reactions, or for 24 h, for reactions larger than 1 ml. Large-scale enzymatic reactions were purified using solid-phase extraction (SPE) with a Phenomenex Strata C18-E reverse phase … TīmeklisThe FD 150/E from PROXXON is a Precision Lathe for metal working, longitudinal turning, boring, taper turning, parting and drilling. A 2-stage belt drive and additional …

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Tīmeklis2024. gada 14. janv. · Europe PMC is an archive of life sciences journal literature. TīmeklisWe characterized the protease domain (LahT150) of a transporter from a lanthipeptide biosynthetic operon in Lachnospiraceae and demonstrate that this protease can remove the leader peptide from a diverse set of peptides. The 2.0 A♂ resoln. crystal structure of the protease domain in complex with a covalently bound leader peptide demonstrates ... dreamland cafe reservations

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"TīmeklisLahT150, which cleaved the peptide at the GGYD sequence (Figure 1E). Overall structures of ThuS and SunS To provide a molecular framework for understanding substrate speciﬁcity, we determined the crystal structures of SunS and ThuS. Initial efforts focused on structure determination of SunS bound to UDP-Glc. " - Laht150

Laht150

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Tīmeklis2024. gada 14. janv. · For the LahT150 digest, 8 μl of purified LahT150 (ref. 90) at a concentration of 20 μM was added at a 1:10 ratio to 72 μl of approximately 200 μM of … TīmeklisWe characterized the protease domain (LahT150) of a transporter from a lanthipeptide biosynthetic operon in Lachnospiraceae and demonstrate that this protease can …

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Tīmeklis2024. gada 14. janv. · Remarkably, LahT150 was able to cleave the leader peptide from a group of peptides encoded in Azospirillum sp. B510 (Figure 4C and Figure … Tīmeklis2024. gada 14. janv. · For the LahT150 digest, 8 μl of purified LahT150 (ref. 90) at a concentration of 20 μM was added at a 1:10 ratio to 72 μl of approximately 200 μM of spun-concentrated protein in glass MS vial ...

Tīmeklis2024. gada 18. aug. · Abstract. Leader peptide removal at a double glycine motif by a protease (LanT) is the final step in the biosynthesis of class II lanthipeptides. LanTs … TīmeklisExperimental support for this revised hypothesis was obtained by reaction of the ThuA peptide with the C39 protease domain LahT150, which cleaved the peptide at the GGYD sequence ( Figure 1E). ...

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Tīmeklis2024. gada 25. jūn. · The elution fraction from the Ni-NTA column was diluted two-fold with 100 mM Tris pH 8.0 and an aliquot of 100 μL of the protease LahT150 (500 μM) purified as previously described, 37 was added to cleave the leader peptide from ProcA 2.1, 2.8 and 2.10. The cleavage reactions were incubated overnight at room …

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