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WebHomoserine dehydrogenase (HSD; E.C. 1.1.1.3) catalyzes the reversible conversion of L-aspartate-β-semialdehyde (L-ASA) to L-homoserine (L-HSE) ().The oxidation of L-ASA to L-HSE is an important step in the aspartate pathway involved in the biosynthesis of L-threonine, L-methionine, and L-isoleucine [].L-ASA is the precursor for the synthesis of L … Webwater for 3 min to inactivate the proteases. The mixture was centrifuged at 8,000×g for 25 min using Supra 22K (Hanil Science Industrial, Incheon, Korea). The supernatant was stored at –20℃ until use. Degree of hydrolysis DH was measured using soluble protein as an indicator. In other words, protein concentration of the supernatant of the parks \u0026 recreation in katy
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