Thp1 antibody
WebDec 1, 2000 · TAK1 mediates activation of p38 and IKKβ by LPS or IL-1 in THP-1 cells. (A) LPS or IL-1 activates p38 in a TAK1-dependent manner. Cells were stimulated for 30 min with the indicated amount of stimuli and p38 was immunoprecipitated with monoclonal anti-p38 α antibodies for kinase assay. (B) Activation of p38 by TNF-α is independent of TAK1. WebThus, we next investigated whether the enhanced migration of THP-1 cells to conditioned medium is dependent on Ccr2. To this end, we disabled Ccr2 using an anti-Ccr2 antibody and performed a transwell migration assay. Blockade of Ccr2 with an anti-Ccr2 antibody appreciably diminished migration of THP-1 cells to conditioned medium (Figure 3f).
Thp1 antibody
Did you know?
WebOnce we have a working reagent, we test the isotype control in tandem with several experimental antibodies in multiple cell types (J774, BV-2, Jurkat, THP-1, and TK-1) and … WebRetroviral production and transduction were performed as described earlier. 11, 18 Human AML cell lines Molm13, MV4-11, KG-1, ME-1, Kasumi, TF-1, NB4, HL60, THP-1, and MO7-E …
WebOct 24, 2024 · THP-1 cells were co-transfected with the human ENPP1 expression plasmid, the ... (Promega). The cell lysates were then immunoblotted with an anti-ENPP1 antibody … WebMonoclonal Antibody for studying IRG1. Cited in 5 publications. Validated for Western Blotting, Immunoprecipitation. Highly specific and rigorously validated in ... Western blot …
WebCustom Monoclonal Antibodies; CellPower™ Assay Cell Line Development; DIAGNOSTICS Hot! COVID-19. cPass™ Technology . Rapid Tests. Resource . News ... We examined the gene and protein regulating effects on THP-1 human macrophages by repeated exposure to therapeutically relevant concentrations of nitroglycerin (NTG) and possible involvement ... WebTherefore, targeting CD47 may be a novel strategy for cancer immunotherapy, and a variety of anti-CD47 antibodies have appeared, such as humanized 5F9 antibody, B6H12 antibody, ZF1 antibody, ... Wang Y, Yin C, Feng L, et al. Ara-C and anti-CD47 antibody combination therapy eliminates acute monocytic leukemia THP-1 cells in vivo and in vitro.
WebTo get M1-macrophages: 24-48h of 50ng/ml IFNg and 50 ng/ml LPS. To get M2-macrophages: 24-48h of IL-4 and IL-13 (25 ng/ml both). In summary, when I go to flow …
WebApr 12, 2024 · We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. crowdfunding taxabilityWebThe results demonstrated the existence of a higher near-wall concentration and a longer residence time of the monocytic analog THP-1 cells near the step and the reattachment point. THP-1 cells showed prominent adhesion to ECs pretreated with TNFα in the regions near the step and the reattachment point, but they showed virtually no adhesion to un … building a cybersecurity cultureWebASC knockout or knockdown in THP-1 cells. Canonical inflammasome signaling in THP1-KO-ASC cells. ASC (apoptosis-associated speck-like protein containing a CARD domain, also … crowdfunding sites for educationWebAtrial natriuretic peptide (ANP), a cardiovascular hormone, elicits different biological actions in the immune system. Aim of the present study was to investigate in THP-1 monocytes the ANP effect on hydrogen peroxide (H2O2)-induced Reactive Oxygen Species (ROS), cell proliferation and migration. building a cyber security companyWebApr 30, 2024 · The PVDF membrane was then sequentially probed with primary antibodies overnight at 4° C and horseradish peroxidase (HRP)-conjugated secondary antibody for 1 h at RT. Blots were developed with enhanced chemiluminescence (ECL) western blot substrate (Thermo Fisher Scientific, USA) ... THP-1 cells (5-6 × 10 6 cells/ml ... crowdfunding sites for ukraineWeb5 IRF1) or alternative activation markers (CCL17, ALOX15, CCL26, TGM2, CD200R and CD206).Similar conditions can be used for polarizing PMA-primed THP-1 cells with IL-10 … building a cyber security scorecardWebJul 16, 2024 · Answer From Todd B. Nippoldt, M.D. Thyroid peroxidase (TPO) is an enzyme normally found in the thyroid gland. TPO plays an important role in the production of … crowd funding singapore